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Single non-lethal doses of chloroform (CHCl3), dichlorobromomethane (CHCl2Br), dibromochloromethane (CHClBr2), or bromoform (CHBr3 )were administered to male rats. Routes of exposure including single intraperitoneal (ip) and subcutaneous (sc) injection were used in order to permit comparison of severity of THM effects and renal toxicity was assessed at varied times following treatment. On an equimolar basis, sc administration of CHCl3 (either 12 or 3mmoles/kg) is more effective at increasing KW/BW than ip CHCl3 treatment. Plasma urea nitrogen (BUN) following ip THM injections are markedly increased with all four THM at 24 hours post treatment. BUN response to CHCl2Br and CHClBr2, treatment remains elevated at 48 hours post administration, but CHCl3 and CHBr3-effected BUN levels have essentially returned to those of vehicle control. THM sc treatment results in a BUN response similar to that seen following ip treatment, with only the time course being different. With the exception of CHBr3, sc and ip-treatments appear to be equally effective in evoking absolute BUN elevations. These results suggest that THM administration induce renal toxicity dependent upon the route of exposure.
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